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| Kato, Kentaro; Sugi, Tatsuki; Takemae, Hitoshi; Takano, Ryo; Gong, Haiyan; Ishiwa, Akiko; Horimoto, Taisuke; Akashi, Hiroomi. |
| Background: Toxoplasma gondii is an obligate intracellular parasite of the phylum Apicomplexa and a major pathogen of animals and immunocompromised humans, in whom it causes encephalitis. Understanding the mechanism of tachyzoite invasion is important for the discovery of new drug targets and may serve as a model for the study of other apicomplexan parasites. We previously showed that Plasmodium falciparum expresses a homolog of human calcium calmodulin-dependent protein kinase (CaMK) that is important for host cell invasion. In this study, to identify novel targets for the treatment of Toxoplasma gondii infection (another apicomplexan parasite), we sought to identify a CaMK-like protein in the T. gondii genome and to characterize its role in the... |
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Palavras-chave: Calcium calmodulin-dependent protein kinase homolog; GAP45; Phosphorylation; T. gondii CaMK-related kinase; Toxoplasma gondii. |
| Ano: 2016 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4447 |
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| Murata, Yuho; Sugi, Tatsuki; Weiss, Louis M.; Kato, Kentaro. |
| Drug treatment for toxoplasmosis is problematic, because current drugs cannot eradicate latent infection with Toxoplasma gondii and can cause bone marrow toxicity. Because latent infection remains after treatment, relapse of infection is a problem in both infections in immunocompromised patients and in congenitally infected patients. To identify lead compounds for novel drugs against Toxoplasma gondii, we screened a chemical compound library for anti-Toxoplasma activity, host cell cytotoxicity, and effect on bradyzoites. Of 878 compounds screened, 83 demonstrated > 90% parasite growth inhibition. After excluding compounds that affected host cell viability, we further characterized two compounds, tanshinone IIA and hydroxyzine, which had IC50 values for... |
| Ano: 2017 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4449 |
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| Adeyemi, Oluyomi Stephen; Murata, Yuho; Sugi, Tatsuki; Kato, Kentaro. |
| This study evaluated the anti-Toxoplasma gondii potential of gold, silver, and platinum nanoparticles (NPs). Inorganic NPs (0.01-1,000 mu g/mL) were screened for antiparasitic activity. The NPs caused >90% inhibition of T. gondii growth with EC50 values of <= 7, <= 1, and <= 100 mu g/mL for gold, silver, and platinum NPs, respectively. The NPs showed no host cell cytotoxicity at the effective anti-T. gondii concentrations; the estimated selectivity index revealed a >= 20-fold activity toward the parasite versus the host cell. The anti-T. gondii activity of the NPs, which may be linked to redox signaling, affected the parasite mitochondrial membrane potential and parasite invasion, replication, recovery, and infectivity potential. Our results... |
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Palavras-chave: Antiparasite; Drug screening; Nanomedicine; Toxoplasmosis. |
| Ano: 2017 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4450 |
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| Adeyemi, Oluyomi Stephen; Murata, Yuho; Sugi, Tatsuki; Han, Yongmei; Kato, Kentaro. |
| Background Toxoplasmosis constitutes a large global burden that is further exacerbated by the shortcomings of available therapeutic options, thus underscoring the urgent need for better anti-Toxoplasma gondii therapy or strategies. Recently, we showed that the anti-parasitic action of inorganic nanoparticles (NPs) could, in part, be due to changes in redox status as well as in the parasite mitochondrial membrane potential. Methods In the present study, we explored the in vitro mode of action of the anti-T. gondii effect of NPs by evaluating the contributions of host cellular processes, including the tryptophan pathway and hypoxia-inducing factor activity. NPs, at concentrations ranging from 0.01 to 200 μg/ml were screened for anti-parasitic activity.... |
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Palavras-chave: Hypoxia Indoleamine 2; 3-dioxygenase Mechanism of action Nanomedicine Toxoplasmosis. |
| Ano: 2017 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4539 |
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